Associate Professor, Colorado State University, Biology Department.
Tenure track position since August 2001.
Department of Biology
Colorado State University
E 416 Anatomy/Zoology Building
Fort Collins, CO 80523, USA
Phone: 970 491 0803
Fax: 970 491 0649
Email: pilon@lamar.colostate.edu

Date of birth: February 26 1963
Place of birth: Utrecht, The Netherlands
Citizenship: The Netherlands/ US permanent resident

Education and training

Postdoctoral Research Training
August 1994 - July 1998: Laboratory of Dr. R. Schekman, Howard Hughes Medical Institute and the Department of Molecular and Cell Biology, University of California at Berkeley.
June 1992-June 1994: Laboratories of Dr. B. de Kruijff and Dr. P. Weisbeek.
Institute for Biomembranes, Utrecht University.

Ph.D.
June 1992: Institute of Molecular Biology, Utrecht University. Advisors: Prof. Dr. P.J. Weisbeek and Prof. Dr. B. de Kruijff.

Masters (There was no bachelors in the Netherlands at the time)
August 1987. Utrecht University.

Positions held
Aug 1987 - June 1992: Graduate Assistant, Utrecht University, The Netherlands, Institute for Molecular Biology
July 1992 - June 1994: Post doctoral researcher, Utrecht University, The Netherlands, Institute for Biomembranes
Aug 1994 - July 1996: Post doctoral researcher, Human Frontier Science Program long term fellowship holder, Laboratory of Dr. R. Schekman, Department of Molecular and Cell Biology, University of California at Berkeley.
Aug 1996 - July 1998: Post doctoral researcher, Howard Hughes Medical Institute, Laboratory of Dr. R. Schekman, Department of Molecular and Cell Biology, University of California at Berkeley.
Aug 1998 - Dec 1998: Research Associate, Biology Dept, Colorado State University
Jan 1999 - March 2001: Special Assistant Professor, Biology Dept, Colorado State University
April 1, 2001 - July 2006: Assistant Professor, Biology Dept, Colorado State University

University Course Instructor at CSU:
BY310 Cell Biology, 4 credits, Spring 1999, Fall 2002, Spring 2004, 2005
+ 1 credit Honors section BY310: fall 2002, Spring 2004, 2005.
NS201 Part 3: Genetics of Development & Basic Immunology , 4 credits
1/3 semester, Spring, 1999
BZ350 Genetics 4 credits, fall, 1999
LSCC102 Introductory biology, 4 credits, fall, 2001,2002, 2004

Present regular CSU Course Assignments:
LSCC102 Attributes of living systems 4 credits (Fall)
BY310 Cell Biology (4 credits (Spring)
BZ 692 Seminar Course 1-2 credits (Spring + Fall)

Research Program: Metal cofactor transport and assembly in chloroplasts.

Research funding:
CSU start up package: $75,000

Past grants
CSU Faculty research grant
Project title: Iron mobilization and Iron Sulfur cluster formation in Chloroplasts: the roles of AtNIFS and AtCRD1. Duration: 1 year (starting July 2001-ending July 2002); award amount: $4,499

NSF MCB-0091163, molecular cell biology-metabolic biochemistry program. PI: Marinus Pilon
Project title: Metal Homeostasis and Tolerance in Plant Chloroplasts- the role of PAA1 and AtCutA.
Duration: 3 years, 4/1/01-3/31/04;
Award amount: $330,000.

NSF-REU supplement cumulative amount: $18,940

USDA-NRI 2003-02148, Co-PI with Elizabeth Pilon-Smits
Project title: Characterization of a NifS-like Chloroplast Protein from Arabidopsis thaliana: Implications for its role in sulfur and selenium metabolism Duration: 2 years, 09/01/03-08/31/05
Award amount $195,000

Active grants

NSF-DBI-0400706, Instrumentation and resources, multi-user equipment program.
Project Title: A Tabletop Ultracentrifuge for Biology
PI: Marinus Pilon, Co-PIs: Daniel Bush, Donald Mykles and Anireddy Reddy
Award amount: $ 32,442
This proposal is for the purchase of a departmental ultra centrifuge.

NSF IBN-0418993
Project Title: Copper delivery in Plant Plastids. PI: Marinus Pilon
09/01/04-08/31/08
Award amount: $ 599,457

USDA-NRI, Co-PI with Elizabeth Pilon-Smits
Project title: Characterization of a NifS-like Chloroplast Protein from Arabidopsis thaliana: Implications for its role in sulfur and selenium metabolism. (renewal of USDA-NRI 2003-02148)
09/01/05-08/31/08
Award amount: $ 362,000

Honors and Fellowships:
August 1994-August 1996: International Human Frontier Science Program Organisation (HFSPO) Postdoctoral research fellowship. Award: $90,000.

January 1996: NVBMB annual award (Netherlands Society for Biochemistry and Molecular Biology). Award amount: Fl 3500 (Dutch guilders, 1 Fl ~ 0.5 USD)

Presentations and Meetings:

Advanced Course Lecturer:
Advanced Course on Plant Biotechnology, Organized by the Institute for Biotechnology Studies Delft/ Leiden, Leiden, The Netherlands (June 1993). Lecture title: "Protein sorting in plant cells."
Dynamics of Membrane Assembly, NATO/FEBS sponsored advanced course, Cargese, Corsica, France (June 1991). Lecture title: "Protein transport into chloroplasts."
Protein-Lipid interactions and molecular aspects of protein insertion and translocation in membranes, FEBS advanced course, Utrecht (January 1989). title: "In vitro analysis of protein import into plastids."

Presentations at international meetings:
13th International Symposium on Iron Nutrition and interactions in Plants. Montpellier, France, July 2006. Oral presentation.
2nd Pan American Plant Transporter Conference, South Padre Island, TX, May 2006, oral presentation
ASPB annual meeting 2005, Mini-symposium speaker and chair, Seattle, July 2005
31st Steenbock symposium, Fe-S clusters, Madison Wisconsin, May 2005, oral presentation
4th Interntional meeting on Copper homeostasis and its disorders. Ischia (Italy) October 2004. Invited speaker.
International Photosynthesis conference, Montreal (Canada) August 2004. Session Co-chair and Speaker
NAIST/RITE Plant Responses to the Environment Symposium. Nara, Japan, November 2003, Invited lecture.
Colloquium in the life sciences CSU, Oct 2003
Plant Biology Dept Seminar, UC Berkeley Oct 2003
ASPB meeting, Honolulu, HI, July 2003, poster
ISPMB, Barcelona, Spain, June 2003, poster
ASPB meeting, Denver, CO (August, 2002) poster
Metals and Cells conference, Canterbury UK (April 2001) Invited lecture
ASPP meeting, San Diego CA (July 2000) poster
Cold Spring Harbor Symposium on Yeast Cell Biology (August 1997) poster
ASCB meeting, San Fransisco CA (December 1996) poster
ASCB meeting, Washington DC (December, 1995) poster
ISPMB meeting, Amsterdam, The Netherlands, (June 1994) Invited talk
International Botany Conference, Yokohama, Japan, (August 1993) Invited talk
Keystone symposium, Taos NM, (February 1993) poster
International Photosynthesis Conference,, Nagoya, Japan, (August 1992)
Invited talk
Dynamics of Membrane Assembly, NATO/FEBS sponsored advanced course,
Cargese, Corsica, France (June 1991) Invited talk
International Photosynthesis Conference,, Stockholm Sweden, (August 1989) poster

Other invited seminars:
University Of Valencia Spain, June 2005
Universite de Poitiers, France, May 2005
Riken, Yokohama Japan, Invited seminar, November 2003
CSU Colloquium in the Life Sciences (Oct 2003)
Invited seminar UC Berkeley (Oct 2003)
Invited Seminar Kyoto University Japan (Nov 2003)
Invited Seminar Riken Yokohama Japan (Nov 2003)
CSU Horticulture Dept seminar (October 2001)
Kyoto University, Japan (June 2001)
CSU Biology Dept. seminar (March, 2001)
DLO-Wageningen University, The Netherlands (May 2000)
Utrecht University, Institute for biomembranes (May 2000)
Plant Biotechnology Symposium, CSU Fort Collins CO (April 2000)
University College London, UK (April 1997)
Amsterdam Free University, The Netherlands (April 1997)

Services/Committee Work

CSU committees:
CMB graduate committee, CMB graduate program, 2006
Research Committee, CMB graduate program, CSU, June 2000-2002.
CMB steering committee, CMB graduate program, CSU, fall 2001-spring 2002.
Search Committee developmental genetics, 2001
Search Committee developmental biologist, Spring 2003
Executive committee, Biology Department, CSU, August 2002-2004
Biology Dept Seminar Committee: Fall 2003-2006
Biology Dept. Graduate Committee: Fall 2004-2006

Other services to the university:
Judge at CMB spring symposium: Spring 2000, 2001, 2002, 2003, 2004, 2005, 2006
Judge at Biology Symposium: Spring 1999, 2000, 2001, 2002, 2003, 2006
Judge at CSU Undergraduate research and creativity symposium: 1999, 2000, 2001, 2002, 2003, 2004, 2005, 2006
Organizer of Plant SuperGroup (PSG). Since the Spring of 2001. PSG provides a forum for grad students and post-docs and an informal setting for front range plant biology researchers to meet.

Ad hoc peer reviewer:
Research Proposals
National Science Foundation, USDA, BART

Scientific Journals
- The Journal of Biological Chemistry
- FEBS letters
- Planta
- PNAS
- Science
- Functional Plant Biology
- Plant and Soil
- Plant Cell
- Plant Journal
- Photosynthesis research

NSF-Panelist: EAPSI program (East Asia and Pacific Studies Summer Institute)

Memberships of professional societies:
- ASPB: American Society for Plant Biology

Lab personnel under my supervision.
Salah Abdel-Ghany, post-doctoral researcher.
Narayana Murthy, post-doctoral researcher.

Graduate student supervision.
Jason Burkhead (Ph.D candidate, Masters completed in Spring 2001, Ph.D in fall 2004)
Ye Hong (Ph.D candidate)
Katie Gogolin (Ph.D candidate)
Christopher Cohu (Ph.D candidate)

Undergraduate supervision. Students taking part in my research program.
Jason Amsbaugh, spring 2000- spring 2003.
Mike Corraggio Spring 2002
Peter Thomas, Spring 2002
Derek Koloditch, Spring 2002
Jared Bodecker Spring 2002- 2004.
Jonathan Morrill, Spring 2002- 2004.
Chris Lei, Fall 2003-2004
Dyllon Martini, Spring 2004-2005
Lance Wollenberg, Fall 20004-2005

Present collaborators.
Dr Toshiharu Shikanai, Nara, Japan
Dr Lola Penarrubia, Valencia, Spain
Dr Elizabeth Pilon-Smits, CSU

Publications:

Chiang YH, Zubo Y, Tapken W, Kim HJ, Lavanway A, Howard L, Pilon M, Kieber J, Schaller GE. (2012)
Functional Characterization of the GATA Transcription Factors GNC and CGA1 Reveals Their Key Role in Chloroplast Development, Growth, and Division in Arabidopsis.
Plant Physiol. 2012 Jul 17. In press out online

Tapken W, Ravet K, Pilon M. (2012)
Plastocyanin controls the stabilization of the thylakoid Cu-transporting P-type ATPase PAA2/HMA8 in response to low copper in Arabidopsis.
J Biol Chem. 287: 18544-50.

Pilon M (2011)
Moving copper in plants.
New Phytol. 192: 305-7.

Ravet K, Danford FL, Dihle A, Pittarello M, Pilon M (2011)
Spatiotemporal analysis of copper homeostasis in Populus trichocarpa reveals an integrated molecular remodeling for a preferential allocation of copper to plastocyanin in the chloroplasts of developing leaves.
Plant Physiol. 157: 1300-12.

Grant K, Carey NM, Mendoza M, Schulze J, Pilon M, Pilon-Smits EA, van Hoewyk D. (2011)
Adenosine 5'-phosphosulfate reductase (APR2) mutation in Arabidopsis implicates glutathione deficiency in selenate toxicity.
Biochem J. 438: 325-35

Balk J, Pilon M (2011)
Ancient and essential: the assembly of iron-sulfur clusters in plants.
Trends Plant Sci.16:2 18-26.

Pilon M, Ravet K, Tapken W (2011)
The biogenesis and physiological function of chloroplast superoxide dismutases.
Biochim Biophys Acta. 1807: 989-98.

Burkhead JL, Gogolin Reynolds KA, Abdel-Ghany SE, Cohu C, Pilon M (2009) Copper homeostasis. New Phytologist 182: 799-816

Abdel-Ghany SE and Pilon M (2008) MicroRNA-mediated Systemic Down- regulation of Copper Protein Expression in Response to Low Copper Availability in Arabidopsis. Journal of Biological Chemistry 283: 15932–15945

Yamasaki H, Pilon M and Shikanai T (2008) How do plants respond to copper deficiency? Plant Signaling & Behavior 3, 231-232

Jeong J, Cohu C, Kerkeb L, Pilon M, Connolly EL and Guerinot ML (2008) Chloroplast Fe(III) Chelate Reductase Activity is Essential for Seedling Viability under Iron Limiting Conditions. Proc. Natl. Acad. Sci USA 105: 10619-24

Padmavathi L,Ye H, Pilon-Smits EAH, Pilon M (2008) Cysteine desulfurase-mediated sulfur donation pathways in plants and phototrophic bacteria. In: (Dahl C, Hell R, Knaff, D Leustek T, eds) Sulfur metabolism in phototrophic organisms. in Series: Advances in Photosynthesis and Respiration (Govindjee, Series Ed). Springer, Berlin, pp 133-149

Murthy N, Ollagnier-de-Choudens S, Sanakis Y, Abdel-Ghany SE, Rousset C, Ye H, Fontecave M, Pilon-Smits EAH, Pilon M (2007) Characterization of Arabidopsis thaliana SufE2 and SufE3: Functions in Chloroplast Iron-Sulfur Cluster Assembly and NAD Synthesis. Journal of Biological Chemistry 282: 18254-18264

Yamasaki H/Abdel-Ghany SE, Cohu CM, Kobayashi Y, Shikanai T, Pilon M (2007) Regulation of Copper Homeostasis by micro-RNA in Arabidopsis. Journal of Biological Chemistry 282:16369-78 http://www.jbc.org/cgi/reprint/M700138200v1

Cohu CM and Pilon M (2007) Regulation of superoxide dismutase expression by copper availability. Physiologia Plantarum, 129, 747-755

Van Hoewyk D, Abdel-Ghany SE, Cohu C, Herbert S, Kugrens P, Pilon M, Pilon-Smits EAH (2007) The Arabidopsis cysteine desulfurase CpNifS is essential for maturation of iron-sulfur cluster proteins, photosynthesis, and chloroplast development. Proceedings of the National Academy of Sciences USA 104: 5686-5691

Puig S, Mira H, Dorcey E, Sancenon V, Andres-Colas N, Garcia-Molina A, Burkhead JL, Gogolin KA, Abdel-Ghany SE, Thiele DJ, Ecker JR, Pilon M, Penarrubia L (2007) Higher plants possess two different types of ATX1-like copper chaperones. Biochemical and Biophysical Research Communications 354:385-390

Schiavon M, Zhang L, Abdel-Ghany SE, Pilon M, Malagoli M, Pilon-Smits EAH (2007) Variation in copper metabolism in Arabidopsis thaliana accessions Columbia, Landsberg erecta and Wassilewskija. Physiologia Plantarum 129: 342-350

Ye H, Pilon M, Pilon-Smits EAH (2006) Iron Sulfur Cluster Biogenesis in Chloroplasts. New Phytologist 171: 285-292.

Pilon M, Abdel-Ghany SE, Cohu CM, Gogolin KA, Ye H. (2006). Copper cofactor delivery in plant cells. Curr Opin Plant Biol. 9: 256-263.

Pilon Smits EAH, Pilon M (2006) Sulfur metabolism in plastids. In: Wise RR, Hoober JK (Eds) "Advances in Photosynthesis and Respiration - The Structure and Function of Plastids". Kluwer Academic Publishers, Dordrecht, The Netherlands. pp 387-402

Ye H, Abdel-Ghany SE, Anderson TD, Pilon-Smits EA, Pilon M (2006).
CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation. J Biol Chem. 281: 8958-69.

Pilon M, Abdel-Ghany SE, Van Hoewyk D, Ye H, Pilon-Smits EA. (2006)
Biogenesis of iron-sulfur cluster proteins in plastids.
Genet Eng (N Y).27:101-17. Review.

Van Hoewyk D, Garifullina GF, Ackley AR, Abdel-Ghany SE, Marcus MA, Fakra S, Ishiyama K, Inoue E, Pilon M, Takahashi H, Pilon-Smits EA (2005). Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis.
Plant Physiol. 139:1518-28.

Salah E. Abdel-Ghany, Jason L. Burkhead, Kathryn A. Gogolin, Nuria Andrés-Colás, Jared R. Bodecker, Sergi Puig, Lola Peñarrubia, and Marinus Pilon (2005)
AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7
FEBS Letters, 579, 2307-2312

Abdel-Ghany SE, Ye H, Garifullina GF, Zhang L, Pilon-Smits EAH, Pilon M (2005) Iron-Sulfur Cluster Biogenesis in Chloroplasts: Involvement of the Scaffold Protein CpIscA. Plant Physiology, 138, 161-172.

Salah E. Abdel-Ghany, Patricia Müller-Moulé, Krishna K. Niyogi, Marinus Pilon* and Toshiharu Shikanai. (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251 * Corresponding author.

Pilon Smits EAH, Pilon M (2005) Sulfur metabolism in plastids. In: Wise RR, Hoober JK (Eds) "Advances in Photosynthesis and Respiration - The Structure and Function of Plastids". Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 387-402.

Ye H, Garifullina GF, Abdel-Ghany S, Zhang L, Pilon-Smits EAH, Pilon M (2005). The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin. Planta, 220, 602-608

Wangeline AL, Burkhead JL, Hale KL, Lindblom SD, Terry N, Pilon M, Pilon-Smits EA. (2004) Overexpression of ATP sulfurylase in Indian mustard: effects on tolerance and accumulation of twelve metals. J Environ Qual. 33, 54-60.

Burkhead J., Abdel-Ghany S., Morrill J., Pilon-Smits E.A.H., and Pilon M. (2003). The Arabidopsis thaliana CUTA gene encodes an evolutionarily conserved copper binding protein. Plant J. 34, 856-867

Shikanai, T., Müller-Moulé, P., Munekage, Y., Niyogi, K., and Pilon, M. (2003). PAA1, a P-type ATPase of Arabidopsis, Functions in Copper Transport in Chloroplasts. Plant Cell 15, 1333-1346. (http://www.plantcell.org/cgi/content/full/15/6/1333)

Pilon M., Owen J.D., Garifullina G.F., Kurihara T., Mihara H., Esaki N., and Pilon-Smits E.A.H. (2003). Enhanced Selenium Tolerance and Accumulation in Transgenic Arabidopsis thaliana Expressing a Mouse Selenocysteine Lyase. Plant Physiol. 131, 1250-1257. (http://www.plantphysiol.org/cgi/content/full/131/3/1250)

Bennett L.E., Burkhead J.L., Hale K.L., Terry N., Pilon M., and Pilon-Smits E.A.H. (2002). Analysis of Transgenic Indian Mustard Plants for Phytoremediation of Metal-Contaminated Mine Tailings. J. Environ. Qual. 32: 432-440.

Garifullina G.F., Owen J.D., Lindblom S.-D., Tufan H., Pilon M., and Pilon-Smits E.A.H. (2003) Expression of a Mouse Selenocysteine Lyase in Brassica juncea Chloroplasts Affects Selenium Tolerance and Accumulation. Physiologia Plantarum 118, 538-544.

Wangeline A.L., Burkhead J.L., Hale K.L., Lindblom S.-D., Terry N., Pilon M., and Pilon-Smits E.A.H. (2004) Overexpression of ATP Sulfurylase in Brassica juncea: Effects on Tolerance and Accumulation of Twelve Metals. J. Environ. Qual., 33, 54-60

Pilon-Smits EAH, Garifullina GF, Abdel-Ghany SE, Kato S-I, Mihara H, Hale KL, Burkhead JL, Esaki N, Kurihara T, and Pilon M (2002)
Characterization of a NifS-like Chloroplast Protein from Arabidopsis thaliana - Implications for Its Role in Sulfur and Selenium Metabolism. Plant Physiol. 130, 1309-1318.

Hale KL, Tufan HA, Pickering IJ, George GN, Terry N, Pilon M, Pilon-Smits EAH (2002) Anthocyanins Facilitate Tungsten Accumulation In Brassica.
Physiologia Plantarum,116, 351-358.

Pilon-Smits EAH, and Pilon M (2002)
Phytoremediation of Metals Using Transgenic Plants Crit. Rev. Plant Sci. 21:439-456

Pilon-Smits E, and Pilon M (2000)
Breeding mercury-breathing plants for environmental cleanup. Trends in plant Science 5, 235-236

Gillece, P, Pilon M and Römisch, K (2000)
The protein translocation channel mediates glycopeptide export across the endoplasmic reticulum membrane. Proc. Natl. Acad. Sci. USA 97, 4609-4614

Pilon M, and Schekman R (1999).
Protein Translocation: How Hsp70 pulls it off. Cell 97, 679-682

Pilon M, Römisch K, Quach D. and Schekman R (1998)
Sec61p serves multiple roles in secretory precursor binding and translocation into the endoplasmic reticulum membrane.
Molecular Biology of the Cell 9, 3455-3473.

Rensink, WA, Pilon M, and Weisbeek PJ (1998)
Domains of a transit sequence required for in vivo import in Arabidopsis Chloroplasts.
Plant Physiology 118, 691-700.

Pilon M, Schekman R and Romisch K. (1997)
Sec61p mediates export of a misfolded secretory protein from the endoplasmic reticulum for degradation. EMBO J. 16, 4540-4548.

De Kruijff B, Pilon M, van 't Hof R, and Demel R (1996)
Lipid-protein interactions in chloroplast protein import.
In: NATO ASI Series Vol H Molecular dynamics of biomembranes (J. Op den Kamp editor.) pp 99-136, Springer-Verlag, Berlin / Heidelberg

Pilon M, Wienk H, Sips W, de Swaaf M, Talboom I, van 't Hof R, de Korte-Kool G, Demel R, Weisbeek P, and de Kruijff B (1995)
Functional domains of the ferredoxin transit sequence involved in chloroplast import.
J. Biol. Chem. 270, 3882-3893.

Forsman C, and Pilon M (1995)
Chloroplast import and sequential maturation of pea carbonic anhydrase: the roles of various parts of the transit peptide. FEBS lett. 358, 39-42.

Bulychev A, Pilon M, Dassen, H, van 't Hof R, Vredenburg W, and de Kruijff B (1995)
Precursor-mediated opening of translocation pores in chloroplast envelopes.
FEBS Lett. 356, 204-206.

Pilon M, America T, van 't Hof R, de Kruijff B, and Weisbeek P (1995)
Protein translocation into chloroplasts.
In: Advances in molecular and cell biology volume title: Membrane protein transport, volume 1 (S.S. Rothman editor), pp 229-255, JAI Press, Greenwich, Connecticut.

Horniak L, Pilon M, van 't Hof R, and de Kruijff B (1993)
The secondary structure of the ferredoxin transit sequence is modulated by its interaction with negatively charged lipids. FEBS Lett. 334, 241-246

van 't Hof R, van Klompenburg W, Pilon M, Kozubek A, de Korte-Kool G, Demel RA, Weisbeek P, and de Kruijff B (1993)
The transit sequence mediates the specific interaction of the precursor of ferredoxin with chloroplast envelope membrane lipids. J. Biol. Chem. 268, 4037-4042.

Pilon M, Rietveld AG, Weisbeek PJ, and de Kruijff B (1992)
Secondary structure and folding of a functional chloroplast precursor protein.
J. Biol. Chem. 267, 19907-19913

Pilon M, Weisbeek PJ, and de Kruijff B (1992)
Kinetic analysis of translocation into isolated chloroplasts of the purified ferredoxin precursor. FEBS Lett. 302, 65-68.

Pilon M, de Kruijff B, and Weisbeek PJ (1992)
New insights into the import mechanism of the ferredoxin precursor into chloroplasts.
J. Biol. Chem. 267, 2548-2556.

Pilon M., de Kruijff, B. and Weisbeek,P.J. (1992)
Molecular aspects of protein translocation into chloroplasts
In: Research in photosynthesis Vol. 3 (N. Murata editor) pp133-140, Kluwer Academic Publishers, Dordrecht.

De Kruijff B, Breukink E, Demel R, van 't Hof R, de Jongh HHJ, Jordi W, Keller RCA, Killian, JA, de Kroon AIPM, Kusters R, and Pilon, M. (1992)
Lipid involvement in protein translocation.
In: New Comprehensive Biochemistry 22, Issue title: Membrane biogenesis and protein targeting (Neupert, W. and Lill, R. eds.) pp 85-101, Elsevier Biomedical Press, Amsterdam.

De Boer D, Hageman J, Pilon M, America T and Weisbeek P (1991)
Protein translocation across the chloroplast envelope membrane.
In: Plant molecular biology (Herrmann, R.G., and Larkins, B., eds.) Plenum Press, New York.

Pilon M, de Boer AD, Knols SL, Koppelman MHGM, van der Graaf, RM, de Kruijff B, and Weisbeek PJ (1990)
Expression in Escherichia coli and purification of a translocation-competent precursor of the chloroplast protein ferredoxin.
J. Biol. Chem. 265, 3358-3361

Hageman J, Baecke C, Ebskamp M, Pilon R, Smeekens S, and Weisbeek P (1990)
Protein import into and sorting inside the chloroplast are independent processes.
Plant Cell 2, 479-494.

Jordi W, Li-Xin Z, Pilon M, Demel RA, and de Kruijff B (1989)
The importance of the amino terminus of the mitochondrial precursor apocytochrome c for translocation across model membranes.
J. Biol. Chem. 264, 2292-2301.

Weisbeek P, Hageman J, De Boer D, Pilon M, and Smeekens S (1989)
Import of proteins into the chloroplast lumen.
J. Cell Sci. Suppl. 11, 199-223.

Pilon M, Jordi W, de Kruijff B, and Demel RA (1987)
Interactions of the mitochondrial precursor protein apocytochrome c with phosphatidylserine in model membranes. A monolayer study.
Biochim.Biophys. Acta 902, 207-216.

Pilon M. and Borstlap A.C (1987)
Kinetics of L-valine uptake in suspension-cultured cells and protoplast-derived cells of tobacco.
Plant Physiol. 84, 737-742